EFFECTS OF LOW-POWER AND DEFOCUSED HIGH-POWER LASER ON THE VIABILITY AND MIGRATION OF MYOBLASTS—A COMPARATIVE IN VITRO STUDY.

Autor: Santos, Lucas Gonçalves, Thomé, Fernanda, Marmora, Belkiss, Schimidt, Tuany Rafaeli, Campos, Paloma Santos, Wagner, Vivian Petersen, Martins, Manoela Domingues
Zdroj: Oral Surgery, Oral Medicine, Oral Pathology & Oral Radiology; Sep2022, Vol. 134 Issue 3, pe184-e184, 1p
Abstrakt: The aim of the present study was to analyze the effect of different photobiomodulation (PBM) therapies in myoblast cell line C2C12 viability and migration. Cells were divided into 9 groups: sham irradiation 10% fetal bovine serum (FBS); sham irradiation 5% FBS; low-level laser 0.1 W; defocused high-power laser (DHPL) 810 1 W; 810 2 W; 980 1 W; 980 2 W; dual 1 W; dual 2 W. To simulate stress conditions, all groups exposed to irradiation were maintained in Dulbecco's Modified Eagle Medium (DMEM) 5% FBS. The impact of therapies on cell viability was assessed through sulforhodamine B assay and on cell migration through scratch assays and time lapse. Myoblast viability was not modified by PBM protocols. All PBM protocols were able to accelerate wound closure 6 and 18 hours after the first irradiation (P <.001). Also, an increase in migration speed, with a more pronounced effect of DHPL using dual wavelength protocol with 1 W, was observed (P <.001). Diverse PBM protocols accelerated C2C12 myoblast migration, with GD1W outstanding as the most effective protocol tested. Benefits from treating muscle injuries with PBM appear to be related to its capacity to induce cell migration without a notable impact on cell viability. [ABSTRACT FROM AUTHOR]
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