Decreased expression of annexin A2 and loss of its association with vascular endothelial growth factor leads to the deficient trophoblastic invasion in preeclampsia.

Autor:	Ruikar, Komal, Aithala, Manjunatha, Shetty, Praveenkumar, Dinesh, Udupi Shastry, Bargale, Anil, Sadashiv, Roshni, Edachery veedu, Sarathkumar, Khode, Vitthal, Neravi, Asha, Patil, Prakash
Předmět:	PREECLAMPSIA diagnosis BLASTOCYST REVERSE transcriptase polymerase chain reaction WESTERN immunoblotting CELL physiology PLACENTA VASCULAR endothelial growth factors MATERNAL mortality INFANT mortality
Zdroj:	Journal of Basic & Clinical Physiology & Pharmacology; Jul2022, Vol. 33 Issue 4, p419-428, 10p
Abstrakt:	Preeclampsia (PE) remains the major cause for maternal and foetal mortality and morbidity. Invasion of endovascular trophoblast and remodelling of spiral artery are crucial actions of normal placental development. Non-fulfilment of these processes plays a leading role in the development of preeclampsia. Vascular endothelial growth factor (VEGF) is produced by extravillous trophoblastic tissue and decidual cell population is a well-known angiogenic growth which plays a fundamental role in placental pathogenesis of PE. Annexin A2 (ANXA2) is a profibrinolytic protein receptor required for plasminolysis, which is an important step in the formation of new blood vessel along with VEGF. Role of ANXA2 is poorly studied in context with human reproductive disease like preeclampsia. The purpose of the present study is to examine the expression and association of VEGF and ANXA2 in the term placentas of pregnancies with and without PE. The study group comprised of placental tissues procured from gestations with PE (n=30) and without (n=20) PE. The expression of VEGF and ANXA2 in the placental villous tissue was evaluated quantitatively by means of IHC, western blotting and reverse transcriptase-polymerase chain reaction (RT-PCR). Our IHC, western blotting and RT-PCR analysis illustrated the significant decrease in the expression of VEGF and ANXA2 in PE group compared with the normotensive control group (p<0.005). We observed statistically significant positive correlation among the expression of ANXA2 and VEGF in placentas of normotensive control group (p<0.0001). The diminished expression of VEGF and ANXA2 in placenta may be associated with the defective angiogenesis and which may possibly play a vital role in PE pathogenesis. [ABSTRACT FROM AUTHOR]
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