| Autor: |
Widowati, Wahyu, Wargasetia, Teresa Liliana, Rahardja, Fanny, Gunanegara, Rimonta F., Priyandoko, Didik, Gondokesumo, Marisca Evalina, Afifah, Ervi, Wijayanti, Cahyaning Riski, Rizal, Rizal |
| Předmět: |
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| Zdroj: |
Asian Pacific Journal of Tropical Biomedicine; Aug2022, Vol. 12 Issue 8, Preceding p343-350, 6p |
| Abstrakt: |
Objective: To evaluate the potential effect of human Wharton’s jelly mesenchymal stem cells (hWJMSCs) on acute respiratory distress syndrome in lipopolysaccharide (LPS)-induced rats. Methods: The hWJMSCs (5×104 /mL, 5×105 /mL, 5×106 /mL) were administered to rats on day 1 and day 8 after being induced by LPS (5 mg/kg body weight). TNF-α levels in the lung and IL18 and IL-1β levels in the serum were measured using ELISA. In addition, caspase-1 expression in lung tissues was quantified using qRT-PCR, and NF-κB and IL-6 expressions were assessed using immunohistochemistry. Results: The hWJMSCs decreased TNF-α levels in the lung and plasma IL-18 and IL-1β levels. Moreover, the hWJMSCs downregulated the expressions of caspase-1, IL-6, and NF-κB in lung tissues. Conclusions: The hWJMSCs can decrease inflammatory markers of acute respiratory distress syndrome in a rat model and may be further investigated for the treatment of acute respiratory distress syndrome. [ABSTRACT FROM AUTHOR] |
| Databáze: |
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