| Abstrakt: |
The thickness of pod reticulation is not only an important criterion of peanut taxonomy, but also an agronomy trait related to peanut mechanical harvesting. To explore the genetic mechanism of reticulation thickness of peanut pods, a novel phenotyping method was developed to determine the reticulation thickness through reconstructing three dimensional (3D) models of pods. Meanwhile, a recombinant inbred line (RIL) population (181 lines) was derived from a cross between Huayu 36 and 6-13 and planted in three environments from 2019 to 2020, including Qingdao, Dongying, and Weihai of Shandong province. Phenotypic data of the RIL population were collected from these three environments. Two related traits, thicknesses of latitudinal and longitudinal protuberant veins (reticulations), had continuous and transgressive distributions in the RIL population, with broad-sense heritablities of 0.92 and 0.91, respectively. Based on a previous high density genetic map, a total of 11 additive QTLs were identified explaining phenotypic variations of 5.21%-11.06%, among which six QTLs were related to thickness of latitudinal protuberant vein and five related to thickness of longitudinal protuberant vein. Two major loci, qLA2 and qLO9 could be detected in more than one environments, with contributing alleles coming from Huayu 36 and 6-13, respectively. A total of 22 pairs of epistatic QTLs involving 34 loci were identified explaining phenotypic variations of 0.55%-4.37%, among which 10 pairs of interactions were related to thickness of latitudinal protuberant vein and 12 pairs were related to thickness of longitudinal protuberant vein. These results provide valuable information for further gene mapping and molecular breeding in peanut. [ABSTRACT FROM AUTHOR] |
