| Autor: |
Pulido, Jorge, García-Durán, Marga, Fernández-Antonio, Ricardo, Galán, Carmen, López, Lissette, Vela, Carmen, Venteo, Ángel, Rueda, Paloma, Rivas, Luis A. |
| Předmět: |
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| Zdroj: |
Journal of Veterinary Diagnostic Investigation; Mar2022, Vol. 34 Issue 2, p190-198, 9p |
| Abstrakt: |
During the COVID-19 pandemic, infection of farmed mink has become not only an economic issue but also a widespread public health concern. International agencies have advised the use of strict molecular and serosurveillance methods for monitoring the SARS-CoV2 status on mink farms. We developed 2 ELISAs and a duplex protein microarray immunoassay (MI), all in a double-recognition format (DR), to detect SARS-CoV2 antibodies specific to the receptor-binding domain (RBD) of the spike protein and to the full-length nucleoprotein (N) in mink sera. We collected 264 mink serum samples and 126 oropharyngeal samples from 5 Spanish mink farms. In both of the ELISAs and the MI, RBD performed better than N protein for serologic differentiation of mink from SARS-CoV2–positive and –negative farms. Therefore, RBD was the optimal antigenic target for serosurveillance of mink farms. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Supplemental Index |
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