| Autor: |
Katsumitsu Shimada, Maya Shimizu, Yuki Ohtani, Roy, Rita R., Satoshi Murakami, Takanaga Ochiai, Hiromasa Hasegawa, Shimada, Katsumitsu, Shimizu, Maya, Ohtani, Yuki, Murakami, Satoshi, Ochiai, Takanaga, Hasegawa, Hiromasa |
| Zdroj: |
Journal of Oral Science; 2021, Vol. 63 Issue 4, p310-314, 5p |
| Abstrakt: |
Purpose: This study aims to delve deeper into the hypothesis that normal salivary gland tissue expresses both protein and mRNA of mammaglobin (MGB).Methods: Formalin-fixed paraffin-embedded samples of submandibular (10), parotid (5), palatal (5) and labial glands (30) salivary glands were immunohistochemically investigated. The labial samples were used to examine the MGB positive ratio (MGB-PR), and localize MGB by double immunofluorescence staining and quantitative mRNA gene expression. Mann-Whitney U and Kruskal Wallis rank-sum test for group comparison, and Spearman's rank correlation coefficient for correlation analysis were used.Results: The distribution of MGB-positive cells was variable throughout samples with significantly higher MGB-PR of acini than ducts (P = 0.00376), and there was no difference when compared based on age (P = 0.0646) and gender (P = 0.245). Besides acinar cells, a number of myoepithelial cells and ductal cells also demonstrated strong MGB reactivity with varying MGB mRNA expression levels in 6 of the 7 samples (with MGB-PR > 20%) tested.Conclusion: This novel study shows that unlike aberrant protein expression in some carcinomas, MGB expression in salivary gland neoplasms represents the nature of original cells, giving a better insight into the neoplasms expressing MGB. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
|
