| Autor: |
Culliton, K.N., Speirs, A.D., Culliton, Kathryn N, Speirs, Andrew D |
| Zdroj: |
Osteoarthritis & Cartilage; Sep2021, Vol. 29 Issue 9, p1362-1369, 8p |
| Abstrakt: |
Objective: The objectives of this study were: first, to compare solute uptake driven by sliding to cyclic uniaxial compression. And secondly, to evaluate the role of the superficial region on passive diffusion to determine if mechanical action is merely overcoming the low permeability of the superficial region or exceeding equilibrium capacity of the tissue.Design: Tests were performed on osteochondral plugs under two types of conditions: cyclic loading (sliding vs axial compression) and unloaded passive diffusion (intact vs superficial zone removed). The articular surfaces were exposed to a fluorescent bath and uptake was quantified from the surface to the subchondral bone using fluorescent microscopy. Primary outcome measures were total mass transfer, mass transfer rate, and surface partition factor.Results: Mass transfer was 2.1-fold higher at 0.5 h for sliding compared to uniaxial compression (p = 0.004). This increased to 4.4-fold at 2 h (p = 0.002). Solute transport for both loading conditions at 2 h had reached or exceeded intact passive diffusion at 12 h. Total mass transport and mass transport per hour was higher in samples without the superficial region compared to intact samples at equilibrium. Rate of mass transfer was not declining for samples subject to sliding indicating solute uptake induced by sliding would exceed passive tissue capacity.Conclusions: These results are the first to quantify solute uptake between two components of joint articulation. The study demonstrates that sliding is a larger driver of solute transport compared to cyclic uniaxial compression. This has implications for cell nutrition, tissue engineering and biochemical signaling. [ABSTRACT FROM AUTHOR] |
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