| Autor: |
Omran, Jihad Ibrahim, A. G., Mohamed Kamel, Anisah N., A. R., Noraina |
| Zdroj: |
International Medical Journal; Feb2021, Vol. 28 Issue 1, p44-47, 4p |
| Abstrakt: |
Introduction: Keratitis caused by the free-living Acanthamoeba is one of the most difficult ocular infections to manage successfully due to the resistance of the organism's cyst stage to most antimicrobial agents. As this condition occurs mainly amongst the contact lens wearers, a contact lens disinfecting solution which is not effective against the Acanthamoeba cyst, may not protect the wearers against its infection. Objective: This study investigates the cysticidal efficacy of Oxysept® hydrogen peroxide system against two environmental isolates of Acanthamoeba from Malaysia. Materials and Methods: Soaking of the Acanthamoeba cyst isolates (TTT1 & TKT19) suspension to Oxysept® hydrogen peroxide system was performed to determine the anti-Acanthamoeba activity of the contact lens disinfecting solution. The soaking times were based on the manufacturer's recommended time (6 hours), 4 hours and over-night soaking for 8 hours. The cysticidal efficacy of the contact lens disinfecting solution was determined by detecting the presence of Acanthamoeba trophozoites on the non-nutrient agar (NNA) plates lawned with heat killed Escherichia coli following disinfection. The emergence of trophozoites from the cysts that had been soaked with Oxysept® hydrogen peroxide system indicates that the system is not effective against Acanthamoeba. Result: Oxysept® hydrogen peroxide system against Acanthamoeba cysts gives varying results. The manufacturer's recommendation of mixing of the disinfectant simultaneously with neutralizing tablets, exhibited no cysticidal effect. However, when the cysts were soaked in hydrogen peroxide solution only without the neutralizing tablet, it exhibited cysticidal effect against Acanthamoeba cysts at all soaking periods. Conclusion: This study concludes that Oxysept® hydrogen peroxide system shows anti-Acanthamoeba activity if it is not used simultaneously with the neutralizing tablet. Therefore the disinfection process should be allowed to take place for some period of time before it is neutralized to achieve the cysticidal effect and thus help prevent Acanthamoeba keratitis. [ABSTRACT FROM AUTHOR] |
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