Protective effects and chemical composition of Corchorus olitorius leaf fractions against isoproterenol-induced myocardial injury through p65NFkB-dependent anti-apoptotic pathway in rats.

Autor:	Alabi, Babatunde, Omobowale, Temidayo, Badejo, Joseph, Adedapo, Adeolu, Fagbemi, Oluwole, Iwalewa, Olugbenga
Předmět:	ANALYSIS of variance ANIMAL experimentation ANTINEOPLASTIC agents APOPTOSIS CELLULAR signal transduction GENE expression ISOPROTERENOL LEAVES MEDICINAL plants MYOCARDIUM PROTEINS RATS STATISTICS T-test (Statistics) PLANT extracts DATA analysis OXIDATIVE stress DATA analysis software DESCRIPTIVE statistics PHARMACODYNAMICS
Zdroj:	Journal of Basic & Clinical Physiology & Pharmacology; Sep2020, Vol. 31 Issue 5, p1-13, 13p
Abstrakt:	Background: The fractions of Corchorus olitorius leaf (COLF) were evaluated against oxidative stress, inflammation and apoptosis in isoproterenol (ISO)-induced myocardial injury (MI) Wistar rats. Methods: The n-hexane, dichloromethane, ethylacetate and ethanol fractions were obtained from COLF extract. Male Wistar strains were randomly grouped into 11 groups (n = 6 in each group), which comprises normal control group, MI control group, 4 fraction groups with two doses (50 and 100 mg/kg) and enalapril (10 mg/kg). The sera were obtained for biochemical studies like AOPP (advance oxidized protein product), CRP (C-reactive protein), LDH (lactate dehydrogenase), CKMB (creatine kinase-MB) and myocardial tissue obtained for GSH, p65NFkB, bax, bcl2, p53 and p65NFkB assays. Results: The subcutaneous administration of ISO increased the serum level of CRP, LDH and CKMB significantly (p < 0.05) and decreased serum AOPP, tissue GSH and p65NFkB (p < 0.05) in the infarction control rats. Pretreatment with COLF and enalapril increased the tissue GSH and p65NFkB levels (p < 0.05) and significantly reduced serum CRP, AOPP, LDH and CKMB. The dichloromethane fraction (CODCM) being the most active was chosen to evaluate the anti-apoptotic effect. CODCM (50 and 100 mg/kg) and enalapril showed a significant (p < 0.05) effect through severe expression of p65NFkB, which correlates with increased bcl2 protein expression, decreased bax protein and p53 expression. Gas chromatography mass spectrometry revealed the presence of 26 compounds in CODCM. Conclusions: From the present study, COLF protected the myocardial tissue against ischemic injury in rats probably via the p65NFkB-dependent anti-apoptotic pathway and attenuation of pro-inflammatory marker level. [ABSTRACT FROM AUTHOR]
Databáze:	Supplemental Index
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