| Autor: |
Mayumi Karimazawa, Shouji Urita, Tetsuro Yamashita, Koichi Suzuki |
| Předmět: |
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| Zdroj: |
Journal of Insect Biotechnology & Sericology; 2019, Vol. 88 Issue 1, p1-6, 6p |
| Abstrakt: |
We developed high-yield degumming methods for preparing sericin from the cocoons of the mulberry silkworm Bombyx mori and the non-mulberry silkworms of Antheraea yamamai, and Cricula trifenestrata. We achieved sericin yields of 54%-55% from B. mori cocoons extracted in 0.24% NaOH, from A. yamamai cocoons extracted in 0.025% NaOH + 0.475% Na2CO3, and from C. trifenestrata cocoons extracted in 0.5% Na2CO3. In all three ex- tracts, analysis of amino acid compositions indicated a high degree of sericin purity and Tricine--SDS-PAGE analysis indicated degradation to heterogeneous polypeptides. Thus, sericin preparations produced from the cocoons of silkworms by high-yield degumming methods offer promise for biomedical and industrial applications. [ABSTRACT FROM AUTHOR] |
| Databáze: |
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