| Autor: |
Yanqiu Wei, Baozhi Yang, Yunlong Li, Yongcheng Duan, Deyu Tian, Baoxiang He, Chuangfu Chen, Wenjun Liu, Limin Yang |
| Předmět: |
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| Zdroj: |
Journal of Veterinary Science; Jul2020, Vol. 21 Issue 4, p1-8, 8p |
| Abstrakt: |
A fluorescent microsphere-based immunochromatographic strip test (FICT) was developed for the rapid, sensitive, and quantitative detection of porcine reproductive and respiratory syndrome virus (PRRSV) antibodies at the pen-side. The assay was based on the formation of a sandwich immune-complex (anti-pig IgG-PRRSV antibodies-NSP7/N), which was validated by a comparison with IDEXX-ELISA using 3325 clinical specimens. The diagnostic specificity, sensitivity, and accuracy of FICT were 97.28, 93.41, and 94.95%, respectively. FICT showed a good correlation with the virus neutralization assay. Overall, a promising pen-side diagnostic tool was developed for the rapid and quantitative detection of PRRSV antibodies within 15 min. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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