| Abstrakt: |
Aim of the study: The aim of this study was to test the influence of, Lentil, sago and naphthalene as a porogens on pore distribution, size and topography as well to test cell growth in the biphasic calcium phosphate (BCP) to improve it as a scaffold for bone tissue engineering. Materials and Methods: A randomized control trial was conducted between January and May 2019 in Alfarabi University College and characterization was performed in university of Technology Baghdad. BCP was synthesized and macroporosity was created using lentil, sago and naphthalene, characterized using X ray diffraction (XRD) and Scanning electron microscopy( SEM). In vitro cytotoxicity was performed. Kruskal-Wallis was used for data analysis, a P value < 0.05 was considered to be significant. Results: XRD results showed two phases Hydroxyapatite and β tricalcium phosphate and no changes occurred after the addition of the porogens. SEM showed well distributed macropores range 200-400 mm as the initial size of the porogens. Naphthalene was the best porogen with open and interconnected pores. All the samples of BCP with different porogens were biocompatible with variable degree. Statistically there was significant difference between the three samples and control P=0.015. Conclusion: Lentil can be used as alternative to naphthalene also sago to a lesser degree. [ABSTRACT FROM AUTHOR] |
