| Autor: |
Arral, Mariah L., Tooley, Christian, Ziino, Emily, Halpern, Jeffrey Mark |
| Předmět: |
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| Zdroj: |
Journal of The Electrochemical Society; 2020, Vol. 167 Issue 2, p1-6, 6p |
| Abstrakt: |
NG-Hydroxy-L-arginine (NOHA) is a stable intermediate product in the urea cycle that can be used to monitor the consumption of L-arginine by nitrous oxide synthase (NOS) to produce nitric oxide (NO) and L-citrulline. Research has implicated the urea cycle in many diseases and NO has cultivated interest as a potential biomarker for neural health. Electrochemical detection is an established, cost-effective method that can successfully detect low levels of analyte concentrations. As one of the few electrochemically active species in the urea cycle, NOHA shows promise as a biomarker for monitoring disruptions in this biochemical process. In this study, we show that NOHA has an oxidation peak at +355 mV vs Ag/AgCl at a glassy carbon electrode. In addition, cyclic voltammetry studies with structural analogs--alanine and N-hydroxyguanidine--allowed us to approximate the oxidation wave at +355 mV vs Ag/AgCl to be a one electron process. Diffusivity of NOHA was found using linear scan voltammetry with a rotating disk electrode and approximated at 5.50 × 10-5 cm2 s-1. Ample work is still needed to make a robust biosensor, but the results here characterize the electrochemical activity and represent principle steps in making a NOHA biosensor. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Supplemental Index |
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