| Autor: |
Masahiro Furuyama, Haruna Nagaoka, Tadashi Sato, Mitsuru Sakaizumi |
| Předmět: |
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| Zdroj: |
Genes & Genetic Systems; Aug2019, Vol. 94 Issue 4, p159-165, 7p |
| Abstrakt: |
Gene-centromere (G-C) mapping provides insight into vertebrate genome composition, structure and evolution. Although medaka fish are important experimental animals, no genome-wide G-C map of medaka has been constructed. In this study, we used 112 interspecific triploid hybrids and 152 DNA markers to make G-C maps of all 24 linkage groups (LGs). Under the assumption of 50% interference, 24 centromeres were localized onto all corresponding medaka LGs. Comparison with 21 centromere positions deduced from putative centromeric repeats revealed that 19 were localized inside the centromeric regions of the G-C maps, whereas two were not. Based on the centromere positions indicated in the G-C maps and those of centromeric repeats on each LG, we classified chromosomes as either biarmed or monoarmed; n = 24 = 10 metacentrics/submetacentrics + 14 subtelocentrics/acrocentrics, which is consistent with the results of previous karyological reports. This study helps to elucidate genome evolution mechanisms, and integrates physical and genetic maps with karyological information of medaka. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Supplemental Index |
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