| Autor: |
Carmona-Fernandes, Diana, Leonardo, Natacha, Casimiro, Renata I., Castro, Alice, Barreira, Sofia C., Santos, Pedro Oliveira, Fernandes, António N., Cortes-Figueiredo, Filipe, Gonçalves, Carolina A., Cruz, Rafael, Fernandes, Mariana L., Ivo, Margarida, Pedro, Luis M., Canhão, Helena, Fonseca, J. E., Santos, Maria José |
| Zdroj: |
Acta Reumatológica Portuguesa; 2019 Special Issue, p23-24, 2p |
| Abstrakt: |
Background and aims: Atherosclerosis and osteoporosis are among the most prevalent diseases and share common risk factors, as well as molecular and pathophysiological mechanisms, frequently occurring in the same individual. We hypothesized that there is an association between vessel and bone behavior and our aim was to understand if atherosclerotic lesions are related with disturbances in bone. Methods: Gene expression of pro-inflammatory cytokines and bone remodeling markers were analyzed in arteries and bones from 45 deceased donors. In 139 patients with advanced atherosclerosis submitted to carotid endarterectomy we explored the associations between gene expression in atherosclerotic plaques and bone mineral density (BMD). Additionally, serum levels of pro-inflammatory cytokines and bone remodeling markers were measured and plaque morphology and immunochemistry evaluated. Comparisons between groups and correlations were performed using parametric and non-parametric tests, as appropriate. When justified multivariable linear regression analyses with backward selection of covariates was performed. Results: The donors' group was composed by 23 men (49.6±17.8 years old) and 22 women (61.1±12.9 years old). The aortas were evaluated macroscopically and in 6 of them (about 13%) calcifications were visible. Gene expression of bone remodeling and inflammatory proteins correlated positively in bone and aorta, independently of donors' age and gender. No association between serum and gene expression levels was found. The carotid endarterectomy patients' group was composed by 95 (68.3%) men, 70.3±8.7 years old and 44 (31.7%) women, 71.5±9.6 years old. 64 patients had low BMD according to WHO definition and among these 3 had a t-score in the range of osteoporosis. The expression of bone formation genes, specifically CBFA1 and OCL, was higher in atheroma plaques from endarterectomized patients with normal BMD comparing with those with low BMD, but we found no differences in the expression of inflammatory markers neither in the serum levels of pro-inflammatory and bone remodeling markers. Immunohistochemical analysis revealed higher CD3 and CD68 scores in patients with normal vs low BMD. Conclusions: We suggest that the relationship between changes observed in bones and vessels in the context of atherosclerotic disease and osteoporosis, may rely on the intrinsic connection between the tissues involved, independently of the progression of the diseases affecting them. [ABSTRACT FROM AUTHOR] |
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