| Autor: |
Le Huyen Ai Thuy, Phucharoen, Krisana, Ideno, Akira, Maruyama, Tadashi, Shinozawa, Takao |
| Předmět: |
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| Zdroj: |
Bioscience, Biotechnology & Biochemistry; Apr2004, Vol. 68 Issue 4, p814-819, 6p, 1 Color Photograph, 3 Diagrams, 1 Chart, 1 Graph |
| Abstrakt: |
Describes the construction of an overproduction of plasmid for the catalese gene, ohktA from an alkali- and halo-tolerant bacterium, Halomonas sp. SK1. Expression and characterization of the chimeric catalase cHktA, produced by the fusion of Mn catalase domain II from Thermus thermophilus to the C-terminus of oHktA; Construction of the expression plasmid for the oHktA; Purification of the catalase and analysis of its activity; Production, purification, and characterization of oHktA. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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