Identification and characterization of a novel bacterial β-glucosidase that is highly specific for the β-1,2-glucosidic linkage of sesaminol triglucoside.

Autor: Sakurai, Akinori, Hongo, Shuntaro, Nair, Arun, Waki, Toshiyuki, Oikawa, Daiki, Nishio, Takuma, Shimoyama, Takefumi, Takahashi, Seiji, Yamashita, Satoshi, Nakayama, Toru
Předmět:
Zdroj: Bioscience, Biotechnology & Biochemistry; Sep2018, Vol. 82 Issue 9, p1518-1521, 4p
Abstrakt: A gene (PSTG2) coding for a novel β-glucosidase belonging to glycoside hydrolase family 3 was identified in the vicinity of the previously identified β-glucosidase gene [sesaminol triglucoside (STG)-hydrolyzing β-glucosidase, PSTG1] in the genome of Paenibacillus sp. strain KB0549. Compared with PSTG1, recombinant PSTG2 more specifically acted on the β-1,2-glucosidic linkage of the STG molecule to transiently accumulate a larger amount of 6-O-(β-D-glucopyranosyl)-β-D-glucopyranosylsesaminol. [ABSTRACT FROM AUTHOR]
Databáze: Supplemental Index