| Autor: |
Rizk, Sanaa A., Metwally, Fateheya M., Elfiky, Asmaa M., Mahmoud, Asmaa A., Badawi, Nadia A., Sharaf, Nevin E., Elhefnawi, Mahmoud M. |
| Předmět: |
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| Zdroj: |
Journal of Genetic Engineering & Biotechnology; Jun2018, Vol. 16 Issue 1, p53-56, 4p |
| Abstrakt: |
Altered miRNAs were associated with cigarette smoking. The study aimed to examine the gene expression level of plasma let-7a among healthy smokers and compared it with the non-smokers. Forty subjects were recruited for the present study and classified into 21 smokers and 19 non-smokers, age, and sex were matched. The software that used to design functional primers was MIRprimer. Quantitative real-time PCR was employed to compare the relative expression of plasma let-7a. Results showed that the level of let-7a was down-regulated in smokers to 0.34fold ( p = 0.006) that of the non-smokers. Plasma let-7a showed an area under curve (AUC) of 0.749 with sensitivity 43% and specificity 100%. In conclusion, plasma let-7a was significantly down-regulated in the smokers, and it might be considered a candidate biomarker to discriminate between smokers and non-smokers. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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