Identification of Single Base-pair Mutation and uidA Gene of Escherichia coli O157:H7 by Peptide Nucleic Acids (PNA) Mediated PCR Clamping.

Autor:	Takiya, Toshiyuki, Futo, Satoshi, Tsuna, Mika, Namimatsu, Takanori, Sakano, Tetsuya, Kawai, Keiichi, Suzuki, Tohru
Předmět:	BACTERIAL genetics ESCHERICHIA coli NUCLEIC acids PEPTIDES POLYMERASE chain reaction GENETIC mutation GENETICS
Zdroj:	Bioscience, Biotechnology & Biochemistry; Feb2004, Vol. 68 Issue 2, p360-368, 9p, 6 Diagrams, 3 Charts, 2 Graphs
Abstrakt:	Discusses the identification of single base-pair mutation and uidA gene of Escherichia coli O157:H7 by peptide nucleic acids (PNA) mediated PCR clamping. Analysis of the kinetic parameters of PNA/DNA association using surface plasmon resonance and melting temperature using fluorescence resonance energy transfer.
Databáze:	Supplemental Index
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