| Autor: |
Slováková, M., Kratochvilová, V., Palarčík, J., Metelka, R., Dvořáková, P., Srbová, J., Munzarová, M., Bilková, Z. |
| Předmět: |
|
| Zdroj: |
Vlakna a Textil; Sep2016, Vol. 23 Issue 3, p193-198, 6p |
| Abstrakt: |
The purpose of the paper is to describe the production and characterization of electrospun chitosan nanofibers and chitosan/TPP nanoparticles as carriers for microbial collagenase. These proteolytic highly active and stable carriers have a potential of advanced bioactive wound dressings. The morphology and the microstructure of nanofibers and nanoparticles were examined using a scanning electron microscopy and dynamic light scattering. Low molecular weight chitosan/TPP nanoparticles prepared at the ratio 2:1 showed a size range of 130 - 142 nm. Specific collagenase activity 0.545 x 10-3 U/mg for chitosan nanofibers and 0.684 x 10-3 U/mg of nanoparticles was quantified using the hydrolysis of a peptidic substrate (Pz-peptide) and is expressed per mass of chitosan biomaterial. Storage stability and reusability of collagenase chitosan nanofibers and collagenase chitosan nanoparticles did not fall below 73% of their original activity. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
|
