| Autor: |
HEGGENDORN, Fabiano Luiz, de Carvalho SILVA, Gabriela Cristina, CARDOSO, Elisama Azevedo, CASTRO, Helena Carla, GONÇALVES, Lúcio Souza, DIAS, Eliane Pedra, de Oliveira Freitas LIONE, Viviane, LUTTERBACH, Márcia Teresa Soares |
| Předmět: |
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| Zdroj: |
Dental Materials Journal; 2016, Vol. 35 Issue 5, p762-768, 7p |
| Abstrakt: |
This study assessed the cell viability of the inoculation vehicle of BACCOR (a combination of sulfate-reducing bacteria plus a culture media for bacteria), a biopharmaceutical product under development for dental use as aid in fractured endodontic file removal from the root canal. Different culture media for bacteria were evaluated: modified Postgate E (MCP-E mod), Modified Postgate E without Agar-agar (MCP-E w/Ag), Postgate C with Agar-agar (MCP-C Ag) and Postgate C without Agar-agar (MCP-C w/Ag). Cytotoxicity was quantified by the MTT test, exposing L929 and Vero cell lines to the vehicles over 24 h. The exposure of L929 cell line to MCP-E w/Ag resulted in biocompatibility (52% cell viability), while the exposure of the Vero kidney line revealed only MCP-E mod as cytotoxic. When diluted, all the vehicles showed biocompatibility with both cell lines. MCP-E w/Ag was the vehicle chosen for BACCOR, because of its biocompatibility with the cells used. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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