Aspects Regarding the In vitro Propagation of 'Royal Gala' Apple Cultivar.

Autor: FIRA, Alexandru, SIMU, Manuela, VLAICU, Bianca, CLAPA, Doina
Předmět:
Zdroj: Bulletin of the University of Agricultural Sciences & Veterinary Medicine Cluj-Napoca. Horticulture; 2015, Vol. 72 Issue 2, p342-349, 8p
Abstrakt: Apple cultivar 'Royal Gala' was multiplied in vitro on starch-gelled MS media supplemented with 0.7 mg/l BAP, which provided intense proliferation of axillary shoots. In order to test the influence of gelling agents upon proliferation rate in this apple cultivar, modified MS media were used, supplemented with 0.7 mg/l BAP and five types gelling agents: fibrous agar, 6.8 g/l; Phytagel + starch, respectively 0.5 + 50 g/l; guar gum, 20 g/l; Psyllium husk, 15 g/l; Phytagel at 2.2 g/l. The highest proliferation rate, 13.44 ± 1.22 was obtained when the media was gelling with the mixture of Phytagel + starch. By using ex vitro rooting and acclimatization in floating perlite, the rooting percentages were between 99.45% and 93.92%, depending on the culture media used in the multiplication stage, from which the axillary shoots were harvested. [ABSTRACT FROM AUTHOR]
Databáze: Supplemental Index