| Autor: |
SHYMANSKYY, I. O., LISAKOVSKA, O. O., MAZANOVA, A. O., RIASNIY, V. M., VELIKY, M. M. |
| Zdroj: |
European Review for Medical & Pharmacological Sciences; Apr2016, Vol. 20 Issue 7, p1379-1383, 5p |
| Abstrakt: |
OBJECTIVE: To evaluate the effectiveness of vitamin D3 and its combined action with vitamin E in the correction of the impairments of phagocyte function caused by chronic glucocorticoid administration. MATERIALS AND METHODS: Phagocytic activity was assessed by the ability of peripheral blood neutrophils and monocytes to capture FITC-labeled Escherichia coli using flow cytometry. Metabolic activity of neutrophils was measured cytochemically as nitro blue tetrazolium (NBT) reduction test. Intracellular reactive oxygen species (ROS) were detected by 2',7'- dichlorofluorescein fluorescence. RESULTS: Prednisolone administration (5 mg/kg b.w., 30 days) was accompanied by vitamin D3 def iciency and decompensat ion of phagocyte function associated with antimicrobial activity (decrease in NBT reduction rate, ROS formation and phagocytic activity). Vitamin D3 co-administration with prednisolone and, to a greater extent, its combination with a-tocopherol (100 IU and 0.5 mg/rat, 30 days respectively) partially restored phagocyte function. CONCLUSIONS: These data suggest that vitamin D3 and a-tocopherol can prevent immunosuppressive effects of prednisolone through elevating the efficacy of oxygen-dependent mechanisms of phagocytosis and increasing the functional activity of phagocytic cells. [ABSTRACT FROM AUTHOR] |
| Databáze: |
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