| Autor: |
Jalloul, Ali H., Szerencsei, Robert T., Schnetkamp, Paul P.M. |
| Zdroj: |
Cell Calcium; Jan2016, Vol. 59 Issue 1, p1-11, 11p |
| Abstrakt: |
The Solute Carrier Family 24 (SLC24) belongs to the CaCA super family of Ca 2+ /cation antiporters and codes for five different K + - dependent Na + - Ca 2+ exchangers (NCKX1-5). NCKX proteins play a critical role in Ca 2+ homeostasis in a wide variety of biological processes such as vision, olfaction, enamel formation, Melanocortin-4-receptor-dependent satiety and skin pigmentation. NCKX transcripts are widely found throughout the brain. In this study we examine the differences between NCKX1-4 in terms of cation dependencies. We measured changes to Ca 2+ influx via the reverse exchange mode while manipulating external Ca 2+ or K + or internal Na + concentrations (External Ca 2+ Dependence, External K + Dependence and Internal Na + Dependence respectively); we also looked at the effect of external Na + /Ca 2+ competition and 3′ 4′- Dichlorobenzamil on the transport of ions in HEK 293 cell lines. A fluorescence based assay was used to determine differences in transport kinetics of the four membrane spanning exchangers using the Michaelis-Menten constant ( K m ). Our results show that there are no significant differences between the NCKX isoforms to explain the variation in the specific expression pattern of these exchangers. [ABSTRACT FROM AUTHOR] |
| Databáze: |
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