| Autor: |
Yuria UMEMURA, Ryosuke MIYAMOTO, Rie HASHIMOTO, Kyoko KINOSHITA, Takuya OMOTEHARA, Daichi NAGAHARA, Tetsushi HIRANO, Naoto KUBOTA, Kiichi MINAMI, Shogo YANAI, Natsumi MASUDA, Hideto YUASA, Youhei MANTANI, Eiko MATSUO, Toshifumi YOKOYAMA, Hiroshi KITAGAWA, Nobuhiko HOSHI |
| Předmět: |
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| Zdroj: |
Journal of Veterinary Medical Science; Dec2015, Vol. 77 Issue 12, p1587-1598, 12p, 5 Color Photographs, 1 Black and White Photograph, 1 Chart |
| Abstrakt: |
Mammalian sexual fate is determined by the presence or absence of sex determining region of the Y chromosome (Sry) in the “bipotential” gonads. Recent studies have demonstrated that both male and female sexual development are induced by distinct and active genetic pathways. Breeding the Y chromosome from Mus m. domesticus poschiavinus (POS) strains into C57BL/6J (B6J) mice (B6J-XYPOS) has been shown to induce sex reversal (75%: bilateral ovary, 25%: true hermaphrodites). However, our B6N-XYPOS mice, which were generated by backcrossing of B6J-XYPOS on an inbred B6N-XX, develop as males (36%: bilateral testis with fertility as well as bilateral ovary (34%), and the remainder develop as true hermaphrodites. Here, we investigated in detail the expressions of essential sex-related genes and histological features in B6N-XYPOS mice from the fetal period to adulthood. The onsets of both Sry and SRY-box 9 (Sox9) expressions as determined spatiotemporally by whole-mount immunohistochemistry in the B6N-XYPOS gonads occurred 2–3 tail somites later than those in B6N-XYB6 gonads, but earlier than those in B6J-XYPOS, respectively. It is possible that such a small difference in timing of the Sry expression underlies testicular development in our B6N-XYPOS. Our study is the first to histologically show the expression and ectopic localization of a female-related gene in the XYPOS testes and a male-related gene in the XYPOS ovaries. The results from these and previous experiments indicate that the interplay between genome variants, epigenetics and developmental gene regulation is crucial for testis development. [ABSTRACT FROM AUTHOR] |
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