Autor: |
Okuyama, Kiyoshi, Shibuya, Susumu, Hamamoto, Tomoki, Noguchi, Toshitada |
Předmět: |
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Zdroj: |
Bioscience, Biotechnology & Biochemistry; May2003, Vol. 67 Issue 5, p989-995, 7p, 1 Diagram, 4 Charts, 3 Graphs |
Abstrakt: |
Develops a procedure for enzymatic synthesis of 2-deoxyguanosine, consisting of the transglycosylation from thymidine to 6-substituted purine (2-amino-6-chloropurine) instead of natural guanine and the conversion of 2-amino-6-chloropurine-2-deoxyriboside to dGuo with bacterial adenosine deaminase. Reaction efficiencies of NdRT-II; Types of NPases needed in the transglycosylation reaction of DAPdR or ACPdR production from dThd by a nucleotide phosphorylase. |
Databáze: |
Supplemental Index |
Externí odkaz: |
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