Interaction between vitamin B6 and source of selenium on the response of the selenium-dependent glutathione peroxidase system to oxidative stress induced by oestrus in pubertal pig.

Autor: Dalto, Danyel Bueno, Roy, Mélanie, Audet, Isabelle, Palin, Marie-France, Guay, Frédéric, Lapointe, Jérôme, Matte, J. Jacques
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Zdroj: Journal of Trace Elements in Medicine & Biology; Oct2015, Vol. 32, p21-29, 9p
Abstrakt: This study aimed to assess the interaction between vitamin B 6 and selenium (Se) for the flow of Se towards the Se-dependent glutathione peroxidase (GPX) system in response to oxidative stress naturally induced by oestrus in a pubertal pig model. At first oestrus, forty-five gilts were randomly assigned to the experimental diets ( n = 9/group): basal diet (CONT); CONT + 0.3 mg/kg of Na-selenite (MSeB 6 0); MSeB 6 0 + 10 mg/kg of HCl-B 6 (MSeB 6 10); CONT + 0.3 mg/kg of Se-enriched yeast (OSeB 6 0); and OSeB 6 0 + 10 mg/kg of HCl-B 6 (OSeB 6 10). Blood samples were collected at each oestrus (long-term profiles), and daily from day −4 to +3 (slaughter) of the fourth oestrus (peri-oestrus profiles) after which liver, kidneys, and ovaries were collected. For long-term profiles, CONT had lower blood Se than Se-supplemented gilts ( p < 0.01) and OSe was higher than MSe ( p < 0.01). Lower erythrocyte pyridoxal-5-phosphate was found in B 6 0 than B 6 10 ( p < 0.01). No treatment effect was observed on GPX activity. For peri-oestrus profiles, treatment effects were similar to long-term profiles. Treatment effects on liver Se were similar to those for long-term blood Se profiles and OSe had higher renal Se concentrations than MSe gilts ( p < 0.01). Gene expressions of GPX1 , GPX3 , GPX4 , and selenocysteine lyase in liver and kidney were greatest in OSeB 6 10 gilts ( p < 0.05). These results suggest that dietary B 6 modulate the metabolic pathway of OSe towards the GPX system during the peri-oestrus period in pubertal pigs. [ABSTRACT FROM AUTHOR]
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