| Autor: |
Feldman I, Feldman GM, Mobarak C, Dunkelberg JC, Leslie KK, Feldman, Irv, Feldman, Gerald M, Mobarak, Charlotte, Dunkelberg, Jeffrey C, Leslie, Kimberly K |
| Zdroj: |
American Journal of Obstetrics & Gynecology; Apr2007, Vol. 196 Issue 4, p394-396, 3p |
| Abstrakt: |
Objective: The objective of the study was to determine whether cross-talk occurs between estrogen receptors (ERs) and nuclear factor-kappa-B (NF-kappaB), to assess the functional consequences of such an ER/NF-kappaB interaction, and to identify other unknown regulatory proteins that may participate in the NF-kappaB transcriptional complex.Study Design: Electromobility gel shifts, reporter gene assays, and mass spectrometry were used to identify proteins interacting with the NF-kappaB deoxyribonucleic acid (DNA) response element.Results: ER and the p65 subunit of NF-kappaB colocalized on DNA. This interaction was inhibitory for ER transcriptional activity. Sequencing of proteins bound to the NF-kappaB/DNA complex identified DNA-modifying enzymes, scaffolding proteins, chaperones, and elements of the nuclear matrix.Conclusion: These studies have identified an inhibitory interaction between estrogen receptors and the p65 subunit of NF-kappaB with implications for estrogen action in pregnancy and cancer. New accessory proteins have also been identified that bind to protein complexes on the NF-kappaB DNA response element. [ABSTRACT FROM AUTHOR] |
| Databáze: |
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