| Autor: |
Santos BA, Roman-Campos D, Carvalho MS, Miranda FM, Carneiro DC, Cavalcante PH, Cândido EA, Filho LX, Cruz JS, Gondim AN |
| Zdroj: |
Phytomedicine; May2011, Vol. 18 Issue 7, p539-543, 5p |
| Abstrakt: |
This study was undertaken to elucidate the effect of the essential oil from Alpinia speciosa (EOAs) on cardiac contractility and the underlying mechanisms. The essential oil was obtained from Alpinia speciosa leaves and flowers and the oil was analyzed by GC-MS method. Chemical analysis revealed the presence of at least 18 components. Terpinen-4-ol and 1,8-cineole corresponded to 38% and 18% of the crude oil, respectively. The experiments were conducted on spontaneously-beating right atria and on electrically stimulated left atria isolated from adult rats. The effect of EOAs on the isometric contractions and cardiac frequency in vitro was examined. EOAs decreased rat left atrial force of contraction with an EC(50) of 292.2±75.7[mu]g/ml. Nifedipine, a well known L-type Ca(2+) blocker, inhibited in a concentration-dependent manner left atrial force of contraction with an EC(50) of 12.1±3.5[mu]g/ml. Sinus rhythm was diminished by EOAs with an EC(50) of 595.4±56.2[mu]g/ml. Whole-cell L-type Ca(2+) currents were recorded by using the patch-clamp technique. EOAs at 25[mu]g/ml decreased I(Ca,L) by 32.6±9.2% and at 250[mu]g/ml it decreased by 89.3±7.4%. Thus, inhibition of L-type Ca(2+) channels is involved in the cardiodepressive effect elicited by the essential oil of Alpinia speciosa in rat heart. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
|
Full Text from ScienceDirect