Chemical modification of peanut conglutin reduces Ig E reactivity but not T cell reactivity in peanut-allergic patients.

Autor:	Hoffen, E., Kleij, H. P. M., den Hartog Jager, C. F., Doorn, W. A., Knol, E. F., Opstelten, D.‐J., Koppelman, S. J., Knulst, A. C.
Předmět:	CONGLUTININ CONGLUTINATION PEANUT allergy IMMUNOGLOBULIN E T cells PHYSIOLOGICAL effects of cytokines IMMUNOBLOTTING ENZYME-linked immunosorbent assay
Zdroj:	Clinical & Experimental Allergy; Dec2014, Vol. 44 Issue 12, p1558-1566, 9p
Abstrakt:	Background Specific immunotherapy for peanut allergy is associated with significant side-effects. Chemically modified allergens may provide a safer alternative. Objective This study aimed to analyse the immunogenicity and allergenicity of modified peanut conglutin. Methods Native peanut conglutin and two modifications thereof were generated ( RA and RAGA). Conglutin-specific T cell lines from 11 peanut-allergic patients were analysed for proliferation and cytokine production. Sera from 14 patients were analysed for Ig E/ Ig G1/ Ig G4 binding by immunoblot and ELISA. IgE reactivity was analysed by direct and indirect basophil activation test ( BAT), in presence and absence of patient plasma or CD32-blocking antibodies. Results T cell proliferation to RA was unchanged, and proliferation to RAGA was reduced compared to native conglutin. Cytokine profiles remained unchanged. Ig E, Ig G1 and Ig G4 binding to RA and RAGA was significantly reduced. In the direct BAT, the relative potency of modified conglutin was decreased in 67% and increased/similar in 33% of the patients. In the indirect BAT, RA and RAGA were 10-100 times less potent than native conglutin. Addition of plasma to the indirect BAT increased the relative potency of modified conglutin in patients with high peanut-specific Ig G levels. This was mediated via blocking of the response to native conglutin, most likely by soluble Ig G, and not via CD32. Conclusion and Clinical Relevance Chemical modification of peanut conglutin by RA retains immunogenicity and reduces allergenicity and may be a promising approach for development of a curative treatment for peanut allergy. In a subgroup of patients, where the reactivity of native conglutin is already partially blocked by Ig G, the effect of the modification of conglutin is less pronounced. [ABSTRACT FROM AUTHOR]
Databáze:	Complementary Index
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