| Autor: |
Stahl, Evi, Martin, Thomas G., Praetorius, Florian, Dietz, Hendrik |
| Předmět: |
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| Zdroj: |
Angewandte Chemie International Edition; Nov2014, Vol. 53 Issue 47, p12735-12740, 6p |
| Abstrakt: |
DNA has become a prime material for assembling complex three-dimensional objects that promise utility in various areas of application. However, achieving user-defined goals with DNA objects has been hampered by the difficulty to prepare them at arbitrary concentrations and in user-defined solution conditions. Here, we describe a method that solves this problem. The method is based on poly(ethylene glycol)-induced depletion of species with high molecular weight. We demonstrate that our method is applicable to a wide spectrum of DNA shapes and that it achieves excellent recovery yields of target objects up to 97 %, while providing efficient separation from non-integrated DNA strands. DNA objects may be prepared at concentrations up to the limit of solubility, including the possibility for bringing DNA objects into a solid phase. Due to the fidelity and simplicity of our method we anticipate that it will help to catalyze the development of new types of applications that use self-assembled DNA objects. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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