| Autor: |
Bouacem, Khelifa, Bouanane-Darenfed, Amel, Boucherba, Nawel, Joseph, Manon, Gagaoua, Mohammed, Ben Hania, Wajdi, Kecha, Mouloud, Benallaoua, Said, Hacène, Hocine, Ollivier, Bernard, Fardeau, Marie-Laure |
| Zdroj: |
Applied Biochemistry & Biotechnology; Nov2014, Vol. 174 Issue 5, p1969-1981, 13p |
| Abstrakt: |
To date, xylanases have expanded their use in many processing industries, such as pulp, paper, food, and textile. This study aimed the production and partial characterization of a thermostable xylanase from a novel thermophilic anaerobic bacterium Caldicoprobacter algeriensis strain TH7C1 isolated from a northeast hot spring in Algeria. The obtained results showed that C. algeriensis xylanase seems not to be correlated with the biomass growth profile whereas the maximum enzyme production (140.0 U/ml) was recorded in stationary phase (18 h). The temperature and pH for optimal activities were 70 °C and 11.0, respectively. The enzyme was found to be stable at 50, 60, 70, and 80 °C, with a half-life of 10, 9, 8, and 4 h, respectively. Influence of metal ions on enzyme activity revealed that Ca enhances greatly the relative activity to 151.3 %; whereas Hg inhibited significantly the enzyme. At the best of our knowledge, this is the first report on the production of xylanase by the thermophilic bacterium C. algeriensis. This thermo- and alkaline-tolerant xylanase could be used in pulp bleaching process. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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