| Autor: |
Bhullar, Ramanjot, Nagarajan, Ragupathi, Bennypaul, Harvinder, Sidhu, Gaganpreet K., Sidhu, Gaganjot, Rustgi, Sachin, von Wettsteinad, Diter, Gill, Kulvinder S. |
| Předmět: |
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| Zdroj: |
Proceedings of the National Academy of Sciences of the United States of America; 9/30/2014, Vol. 111 Issue 39, p14187-14192, 6p |
| Abstrakt: |
Although studied extensively since 1958, the molecular mode of action of the Pairing homeologous 1 (Ph1) gene is still unknown. In polyploid wheat, the diploid-like chromosome pairing is principally controlled by the Ph1 gene via preventing homeologous chromosome pairing (HECP). Here, we report a candidate Ph 1 gene (C-Ph 7) present in the Ph1 locus, transient as well as stable silencing of which resulted in a phenotype characteristic of the Ph1 gene mutants, including HECP, multivalent formation, and disrupted chromosome alignment on the metaphase I (Ml) plate. Despite a highly conserved DNA sequence, the C-Ph1 gene homeologues showed a dramatically different structure and expression pattern, with only the SB copy showing Ml-specific expression, further supporting our claim for the Ph 1 gene. In agreement with the previous reports about the Ph1 gene, the predicted protein of the 5A copy of the C-Ph 1 gene is truncated, and thus perhaps less effective. The 5D copy is expressed around the onset of meiosis; thus, it may function during the earlier stages of chromosome pairing. Along with alternate splicing, the predicted protein of the 5B copy is different from the protein of the other two copies because of an insertion. These structural and expression differences among the homeologues concurred with the previous observations about Ph1 gene function. Stable RNAi silencing of the wheat gene in Arabidopsis showed multivalents and centromere clustering during meiosis I. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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