| Autor: |
Sanderson, Andrew, Mitchell, Jennie E., Minchin, Stephen D., Busby, Stephen J.W. |
| Předmět: |
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| Zdroj: |
FEBS Letters; Jun2003, Vol. 544 Issue 1-3, p199, 7p |
| Abstrakt: |
Previous work has shown that the base sequence of the DNA segment immediately upstream of the −10 hexamer at bacterial promoters (the extended −10 element) can make a significant contribution to promoter strength. Guided by recently published structural information, we used alanine scanning and suppression mutagenesis of Region 2.4 and Region 3.0 of the Escherichia coli RNA polymerase σ70 subunit to identify amino acid sidechains that play a role in recognition of this element. Our study shows that changes in these regions of the σ70 subunit can affect the recognition of different extended −10 element sequences. [Copyright &y& Elsevier] |
| Databáze: |
Complementary Index |
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