An archaeal homing endonuclease I-PogI cleaves at the insertion site of the neighboring intron, which has no nested open reading frame11The nucleotide sequences of the two introns, Pog.S1205 and Pog.S1213, have been deposited to the GenBank/EMBL/DDBJ databases under the accession number AB087402.

Autor: Nakayama, Hitoshi, Morinaga, Yayoi, Nomura, Norimichi, Nunoura, Takuro, Sako, Yoshihiko, Uchida, Aritsune
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Zdroj: FEBS Letters; Jun2003, Vol. 544 Issue 1-3, p165, 6p
Abstrakt: Homing endonucleases (HEs) of the LAGLIDADG family cleave intron/inteinless cognate DNA at, or near, the insertion site (IS) of their own intron/intein. Here, we describe a notable exception to this rule. Two introns, Pog.S1205 (length 32 bp) and Pog.S1213 (664 bp), whose ISs are 8 bp apart, exist within the 16S rRNA gene of the archaeon Pyrobaculum oguniense. Pog.S1213 harbors a nested open reading frame (ORF) encoding a 22 kDa monomeric protein, I-PogI, which contains two LAGLIDADG motifs and has optimal DNA cleavage activity at 90°C. Intriguingly, I-PogI cleaves the Pog.S1205-less substrate DNA in the presence or absence of Pog.S1213. The cleavage site (CS) of I-PogI does not coincide with the IS of Pog.S1213 but with that of Pog.S1205. Thus, I-PogI activity both promotes the homing of its own intron, Pog.S1213, and guarantees co-conversion of the ORF-less intron Pog.S1205. [Copyright &y& Elsevier]
Databáze: Complementary Index
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