| Abstrakt: |
[ Objective ]This research was aimed to investigate the antibacterial activity in vitro induced by water extracts of the Holly Bark combined with antibacterial agents to provide references for the comprehensive development and utilization of Holly Bark. [ Method ]The minimal inhibition concentration (MIC) of water extracts of Holly Bark and antibacterial agents were tested through twice micro-dilution method. Extended spectrum beta-lactamases (ESBLs) bacterial were induced and subcultured by the 1/2MIC of water extracts of Holly Bark combined with antibacterial agents. Fractional inhibitory concentration (FICI) indexes of extracts combined with antibacterial agents were determined by microdilution checkerboard techniques. [ Result ]The MIC of water extracts of Holly bark was 1.0 g/mL. The MIC of ESBLs-producing bacterial decreased significantly after inducing by 0.5g/mL of water extracts of Holly Bark combined with 6 antibacterial agents, such as amoxicillin, levofloxacin, clinafloxacin, sulfamonomethoxine, gatifloxacin, ceftiofur sodium. FICI indexes were 0.375, 0.750, 0.750, 0.515, 0.750 and 0.750, respectively. [ Conclusion ] Antibacterial activities of antibacterial agents against ESBLs-producing bacterial are enhanced significantly by water extracts of Holly Bark, showing additive effect or synergistic activity correlation. [ABSTRACT FROM AUTHOR] |
