| Abstrakt: |
Objectives: In this study, we aimed to evaluate that the effects of N-acetyl-cysteine (NAC), amifostine (AMF) and ascorbic acid (ASC) on methotrexate (MTX)-induced cerebrum and cerebellum damage. Methods: 55 Sprague Dawley rats divided into 5 equal groups. Control, MTX (20 mg/kg/single dose/ip, was carried out 2nd day of experiment), MTX+NAC (50 mg/kg/day/po, 7 days), MTX+AMF (50 mg/kg/day/ip, 7 days), MTX+ASC (10 mg/kg/day/ip, 7 days). At the end of the study cerebrum and cerebellum were removed and routin histological procedures were done. H-E-stained sections were evaluated and histopathological damage score (For cerebrum; perivascular edema, infiltration, congestion, hemorrhage, vacuolization) (For cerebellum; loss of purkinje cell, purkinje cell degeneration, hemorrhage) was calculated (0-3). Sections were examined using a Leica DFC280 light microscope and a Leica Q Win Image Analysis system (Leica Micros Imaging Solutions Ltd., Cambridge, UK). Statistical analysis was carried out using the SPSS 13.0 (SPSS Inc., Chicago, III., USA) statistical program. For comparison Kruskal-Wallis and Mann-Whitney U tests were used. All data are expressed as arithmetic mean±SE. p<0.05 was regarded as significant. Results: Cerebrum and cerebbelum tissues of control group was normal in histological appearance. In cerebrum-MTX group, perivascular edema, infiltration, congestion, hemorrhage, neuronal vacuolization and cerebellum-MTX group, loss of purkinje cell, purkinje cell degeneration, hemorrhage were observed. In treatment groups, histopathological changes were reduced. When compared with the MTX group, significant differences were detected in treatment groups (p<0.05, for all tissues). Conclusion: We concluded that NAC, AMF and ASC treatments are beneficial in MTX-induced cerebrum and cerebellum damage in rats. [ABSTRACT FROM AUTHOR] |
