| Autor: |
Wencelová, M., Váradyová, Z., Mihaliková, K., Guothová, L., Janštová, J., Čertík, M., Homoľová, L., Pristaš, P., Jalč, D., Kišidayová, S. |
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| Zdroj: |
Journal of Applied Microbiology; Oct2014, Vol. 117 Issue 4, p930-939, 10p |
| Abstrakt: |
Aims Enrichment of wheat bran ( WB), corn meal ( CM) and barley flakes ( BF) with the oleaginous fungus Cunninghamella echinulata ( CE) might lead to effective use of these by-products in ruminant nutrition. We examined their effects on rumen fermentation and lipid metabolism. Methods and Results WB, CM and BF substrates without or with brewer's grains ( WBG, CMG, BFG) and enriched with CE were incubated with meadow hay ( MH, 500 : 500, w/w) in rumen fluid in vitro for 24 h. The dry matter of the CE-enriched substrates increased (by 2-4%); however, digestibility decreased ( P < 0·01). Adverse effects of CE-enriched substrates on the rumen ciliate population were observed. Little effect on the rumen eubacterial population was detected by the 16S-polymerase chain reaction/denaturizing gradient gel electrophoresis method. The increase in γ-linolenic acid output in the MH + BFGCE diet (800 : 200, w/w) was accompanied by an increase in rumen biohydrogenation of polyunsaturated fatty acids. Conclusion The diet substrates enriched with the fungus CE were less digestible than the untreated cereal substrates; no appreciable positive effect was observed on rumen fermentation patterns or the eubacterial and ciliate populations. Significance and Impact of the Study The in vitro study showed that adding CE-enriched substrates to ruminant diets is not effective for improving rumen fermentation. [ABSTRACT FROM AUTHOR] |
| Databáze: |
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