| Autor: |
Lassak, Jürgen, Fried, Luitpold, Jung, Kirsten |
| Zdroj: |
BioSpektrum; Sep2014, Vol. 20 Issue 5, p510-513, 4p |
| Abstrakt: |
β-galactosidase encoded by lacZ remains a popular reporter enzyme. Here, we present a convenient tool box to facilitate rapid construction of reporter lacZ fusions. The first tool enables the simple generation of chromosomally encoded reporters within the Escherichia coli lac operon using Red®/ET® recombination. The second tool allows the introduction of lacZ reporter fusions into the chromosome by a single-crossover method. The third tool comprises four pBBR1-based broad-host-range vectors to derive transcriptional and translational lacZ fusions. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
|
Full text from SpringerLink