| Autor: |
Kwon, Sang-Mo, Lee, Jun-Hee, Lee, Sang-Hun, Jung, Seok-Yun, Kim, Da-Yeon, Kang, Song-Hwa, Yoo, So-Young, Hong, Jong-Kyu, Park, Ji-Hye, Kim, Jung-Hee, Kim, Sung-Wook, Kim, Yeon-Ju, Lee, Sun-Jin, Kim, Hwi-Gon, Asahara, Takayuki |
| Předmět: |
|
| Zdroj: |
PLoS ONE; Aug2014, Vol. 9 Issue 8, p1-12, 12p |
| Abstrakt: |
Introduction: Despite the crucial role of endothelial progenitor cells (EPCs) in vascular regeneration, the specific interactions between EPCs and hematopoietic cells remain unclear. Methods: In EPC colony forming assays, we first demonstrated that the formation of EPC colonies was drastically increased in the coculture of CD34+ and CD34− cells, and determined the optimal concentrations of CD34+ cells and CD34− cells for spindle-shaped EPC differentiation. Results: Functionally, the coculture of CD34+ and CD34− cells resulted in a significant enhancement of adhesion, tube formation, and migration capacity compared with culture of CD34+ cells alone. Furthermore, blood flow recovery and capillary formation were remarkably increased by the coculture of CD34+ and CD34− cells in a murine hind-limb ischemia model. To elucidate further the role of hematopoietic cells in EPC differentiation, we isolated different populations of hematopoietic cells. T lymphocytes (CD3+) markedly accelerated the early EPC status of CD34+ cells, while macrophages (CD11b+) or megakaryocytes (CD41+) specifically promoted large EPC colonies. Conclusion: Our results suggest that specific populations of hematopoietic cells play a role in the EPC differentiation of CD34+ cells, a finding that may aid in the development of a novel cell therapy strategy to overcome the quantitative and qualitative limitations of EPC therapy. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
|
