| Abstrakt: |
To develop the strong restorer lines for WA-CMS and Y-CMS and analyse the pyramiding effect of the Rf3 and Rf4 genes, five indica chromosome single segment substitution lines (SSSLs) with the fertility restoration( Rf3) on chromosomes 1 were crossed with 4 SSSLs carrying the Rf4 gene on chromosomes 10 forming 11 F2 and F3 populations by backcrossing and marker-assisted selection(MAS). In the F2 and F3 populations, six double segments pyramiding lines (DSPLs) carrying the genotype Rf3Rf3 / Rf4Rf4 and five secondary SSSLs (SSSSLs) with the Rf3 or Rf4 gene were selected by MAS. The results showed that there were wide differences in restoring abilities for wild abortive( WA) cytoplasmic male sterility( CMS) among the six DSPLs. Of which the restoration abilities of the DSPL04-01/23-10P1,DSPL04-01/23-10P2 and DSPL04-01/23-10P3,carrying the genotype Rf3-4Rf3-4 / Rf4-4Rf4-4,were stronger than that of the DSPL22-01/27-10P with the genotype Rf3-4Rf3-4 / Rf4-3Rf4-3,and the restoring ability of the DSPL22-01/27-10P appeared to be stronger than those of DSPL11-01/14-10P and DSPL22-01/14-10P with the genotype Rf3- 4Rf3-4 / Rf4-2Rf4-2. The length of substituted chromosome segments in the five SSSSLs ranged from 24. 1 cM to 28. 5 cM with an average of 26. 8 cM,and the total length of substituted chromosome segments from them was 133. 9 cM. To reveal the pyramiding effect of the Rf genes,the DSPL04-01/23-10P1 and its corresponding SSSLs were crossed with a typical WA-CMS lines of 'Zhenshan 97A' (ZsA)and a typical Y-CMS line of 'Y-Huanong A' (HnA), respectively. In the F1 populations, the plants carrying the Rf3rf3 / Rf4rf4 genotype were selected and their phenotyping for pollen was evaluated. The restoration ability of the DSPL04-01/23-10P1 was showed to be stronger than that of its corresponding SSSLs, but the difference between them was not significant. Conclusions: There was not interaction effect between the Rf3 and Rf4 genes from different donors in the DSPLs. [ABSTRACT FROM AUTHOR] |
