| Autor: |
Chesnoy, Sophie, Pui-Yan Lee, Huang, Leaf |
| Předmět: |
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| Zdroj: |
Pharmaceutical Research; Mar2003, Vol. 20 Issue 3, p345, 6p, 12 Color Photographs, 1 Graph |
| Abstrakt: |
Purpose. To evaluate the biologic effect of direct cutaneous TGF-β1 gene delivery on impaired wound healing models using genetically diabetic mice. Methods. Diabetic mice (C57BKS.Cg-m +/+ Leprdb female mice) with 1 cm × 1 cm excisional wounds were intradermally injected with 60 µg of plasmid DNA encoding TGF-β1 gene. The wound closure was measured up to 14 days postwounding. At days 7 and 14 postwounding, sections of skin were taken for hematoxylin and eosin and Masson's trichome staining to examine the morphology and collagen deposition. The cell proliferation and TGF-β1 gene expression were studied using immunohistochemical stainings for 5-bromo-2-deoxyuridine and for TGF-β1. Results. A higher cell proliferation rate and a denser and more organized new extracellular matrix were observed in the treated wound site. Complete wound closure was detected as early as 7 days for TGF-β1-treated group in comparison with 11-14 days for the untreated, control plasmid DNA- and PBS-treated groups. Conclusion. A single intradermal injection of TGF-β1 plasmid DNA was sufficient to enhance wound healing. This approach represents a new strategy that may be applied to the treatment of excisional wounds in human diabetic patients. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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