Presence and function of a thick mucous layer rich in polysaccharides around Bacillus subtilis spores.

Autor: Faille, Christine, Ronse, Annette, Dewailly, Etienne, Slomianny, Christian, Maes, Emmanuel, Krzewinski, Frédéric, Guerardel, Yann
Předmět:
Zdroj: Biofouling; 2014, Vol. 30 Issue 7, p845-858, 14p
Abstrakt: This study was designed to establish the presence and function of the mucous layer surrounding spores ofBacillus subtilis. First, an external layer of variable thickness and regularity was often observed onB. subtilisspores. Further analyses were performed onB. subtilis98/7 spores surrounded by a thick layer. The mechanical removal of the layer did not affect their resistance to heat or their ability to germinate but rendered the spore less hydrophilic, more adherent to stainless steel, and more resistant to cleaning. This layer was mainly composed of 6-deoxyhexoses, ie rhamnose, 3-O-methyl-rhamnose and quinovose, but also of glucosamine and muramic lactam, known also to be a part of the bacterial peptidoglycan. The specific hydrolysis of the peptidoglycan using lysozyme altered the structure of the required mucous layer and affected the physico-chemical properties of the spores. Such an outermost mucous layer has also been seen on spores ofB. licheniformisandB. clausiiisolated from food environments. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index