| Autor: |
Bock, C. H., Endalew, T. T., Biswas, B. K., Yadav, A. K., Sitther, V., Hotchkiss, M. W., Stevenson, K. L., Wood, B. W., Klopfenstein, N. |
| Předmět: |
|
| Zdroj: |
Forest Pathology; Aug2014, Vol. 44 Issue 4, p266-275, 10p |
| Abstrakt: |
Fusicladium effusum infects pecan causing yield loss, but no information is available on the genetic diversity of F. effusum. Randomly amplified polymorphic DNAs ( RAPDs) and universally primed polymerase chain reaction ( UP- PCR) were compared to detect polymorphisms on a group of 20 isolates of F. effusum from 11 geographical locations in the southeastern USA. Two tests (run 1 and 2) of both the RAPD and UP- PCRs were conducted to assess the repeatability of the methods, and the markers scored on agarose gels. In addition, the UP- PCR markers from run 1 were scored using an automated capillary system. Both RAPDs and UP- PCR markers detected a high level of polymorphism among the scored markers (92 and 91% of RAPD markers, and 86 and 87% of manually scored UP- PCR markers in run 1 and 2 were polymorphic, respectively; 93% of UP- PCR markers were polymorphic when scored using the automated system). Unweighted paired group method of arithmetic averages ( UPGMA) analysis showed both RAPDs and UP- PCR markers individually identified each isolate, producing three groupings, but only the groupings based on run 1 and 2 of the UP- PCR contained the same isolates. Bootstrap analysis based on the Dice coefficient produced phenograms from the UP- PCR data with weak to moderate node support (≥54) for the primary branch, but no support for the RAPDs data (≤34). A Mantel test of runs 1 and 2 using RAPDs or UP- PCR showed good agreement ( r = 0.8761 and 0.8289, p < 0.0001), but poor agreement between RAPDs and UP- PCR. UP- PCR results based on the interisolate Dice coefficients showed a weak to strong association with distance. Based on these results, both RAPDs and UP- PCR markers were capable of demonstrating polymorphisms and identifying relationships among isolates of F. effusum; however, UP- PCR markers appear to be more reliable. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
|
Plný text