| Autor: |
Mesquíta Braga, Raissa, Ferreira Santana, Mateus, Veras da Costa, Rodrigo, Brommonschenkel, Sergio Herminio, des de Araujo, Elza Fernan, Vieira de Queiroz, Marisa |
| Předmět: |
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| Zdroj: |
Mycologia; Jul/Aug2014, Vol. 106 Issue 4, p629-641, 13p |
| Abstrakt: |
Transposable elements are ubiquitous and constitute an important source of genetic variation in addition to generating deleterious mutations. Several filamentous fungi are able to defend against transposable elements using RIP(repeat-induced point mutation)-like mechanisms, which induce mutations in duplicated sequences. The sequenced Colletotrichum graminicola genome and the availability of transposable element databases provide an efficient approach for identifying and characterizing transposable elements in this fungus, which was the subject of this study. We identified 132 full-sized Tc1-Mariner transposable elements in the sequenced C. graminicola genome, which were divided into six families. Several putative transposases that have been found in these elements have conserved DDE motifs, but all are interrupted by stop codons. An in silico analysis showed evidence for RIP-generated mutations. The TCgl element, which was cloned from the Brazilian 2908 m isolate, has a putative transposase sequence with three characteristic conserved motifs. However, this sequence is interrupted by five stop codons. Genomic DNA from various isolates was analyzed by hybridization with an internal region of TCgl. All of the isolates featured transposable elements that were similar to TCgl, and several hybridization profiles were identified. C. graminicola has many Tc1-Mariner transposable elements that have been degenerated by characteristic RIP mutations. It is unlikely that any of the characterized elements are autonomous in the sequenced isolate. The possible existence of active copies in field isolates from Brazil was shown. The TCgl element is present in several C. graminicola isolates and is a potentially useful molecular marker for population studies of this phytopathogen. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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