| Autor: |
Chumyim, Porramate, Rijiravanich, Patsamon, Somasundrum, Mithran, Surareungchai, Werasak |
| Zdroj: |
BioNanoScience; Sep2014, Vol. 4 Issue 3, p240-250, 11p |
| Abstrakt: |
We have modified carboxylic acid-functionalised multi-walled carbon nanotubes (MWNTs) with multilayers of tyrosinase/polyallylamine using a layer-by-layer process. Based on optical determination, each tyrosinase deposition coated approximately three layers of enzyme onto the nanotubes. Based on kinetic analysis of the steady-state current of the modified MWNTs to catechol, the minimum value of k/ K for the immobilised enzyme was 3.9 × 10 cm s mol ± 0.5 × 10 cm s mol ( n = 3), which is one order of magnitude less than the value for the enzyme in solution. As an illustration of an immunoassay application, the modified MWNTs were coated with streptavidin and then used as electrochemical labels for biotin-tagged anti-rabbit Salmonella polyclonal antibodies. Following optimisation of enzyme layer number, pH, incubation time and electrochemical parameters, heat-killed Salmonella Typhimurium cells were calibrated across the range 1 CFU ml to 10 CFU ml by direct enzyme-linked immunosorbent assay (ELISA) with a limit of detection (signal/noise = 3) of approx. 340 CFU ml. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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