| Autor: |
Hussein, Nawfal R., Muhammed, Ary H., Al-Dabbagh, Samim, Abdulkareem, Warveen L., Assafi, Mahde S. |
| Předmět: |
|
| Zdroj: |
International Journal of Pure & Applied Sciences & Technology; 2014, Vol. 21 Issue 2, p8-10, 3p |
| Abstrakt: |
A single allele-specific PCR targeting the G88A polymorphism was found to be specific for the detection of USA300 community acquired methicillin resistant Staphylococcus aureus (CA-MRSA). This study aims to determine the prevalence of USA300 in Kurdistan region-Iraq based on this method. We have access to 52 frozen CA-MRSA strains isolated from clinical samples and 10 frozen CA-MRSA strains isolated from healthy carriers. These strains have already been genotyped for SCCmec and PVL gene. These strains were examined for the nuc and mecA genes, then PCR targeting the G88A polymorphism was performed to determine the prevalence of USA300 strain in the community. Two of the clinical samples typed as USA300 CA-MRSA both typed PVL positive and SCCmec type IV. One of the strains isolated from healthy carrier typed as USA300, SCCmec type IV and PVL positive. This is the first study investigating USA300 CA-MRSA prevalence in Kurdistan region in Iraq. USA300 isolates are now epidemic in the USA and are gaining ground around the world. Therefore, actions should be taken to minimize the emergence and transmission of these strains. This method is a cost-effective, a high-throughput, and a rapid method for the detection of USA300 CA-MRSA which offers the opportunity to study such a clinically important strain especially in developing countries. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
|
