| Autor: |
Matveeva, Evgenia G., Nelen, Marina I., Lobanov, Oleg I., Savitsky, Alexander P. |
| Předmět: |
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| Zdroj: |
Journal of Fluorescence; Jan2003, Vol. 13 Issue 1, p79, 15p, 3 Charts, 17 Graphs |
| Abstrakt: |
The antigen-antibody interaction between coproporphyrin I and anti-coproporphyrin antibodies was studied by a fluorescence method in water and a reverse micellar system: n-octane/Aerosol OT. Coproporphyrin fluorescence was quenched, and coproporphyrin emission maximum was shifted to the long-wavelength region after binding to the antibodies or Fab'-fragments. The mechanism of this quenching is static, most probably, by a tryptophan residue (or maybe lysine or methionine). Apparent dynamic quenching, in this case, arises from protein backbone motion. A special kind of antibody Fab'-Fab' dimerization was proposed. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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