| Autor: |
Vogt, Leonie M., Meyer, Diederick, Pullens, Gerdie, Faas, Marijke M., Venema, Koen, Ramasamy, Uttara, Schols, Henk A., de Vos, Paul |
| Předmět: |
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| Zdroj: |
Journal of Nutrition; 7/1/2014, Vol. 144 Issue 7, p1002-1008, 7p |
| Abstrakt: |
Dietary fiber intake is associated with lower incidence and mortality from disease, but the underlying mechanisms of these protective effects are unclear. We hypothesized that β2 →-fructan dietary fibers confer protection on intestinal epithelial cell barrier function via Toll-like receptor 2 (TLR2), and we studied whether β2→-fructan chain-length differences affect this process. T84 human intestinal epithelial cell monolayers were incubated with 4 β2→-fructan formulations of different chain-length compositions and were stimulated with the proinflammatory phorbol 12-myristate 13-acetate (PMA). Transepithelial electrical resistance (TEER) was analyzed by electric cell substrate impedance sensing (ECIS) as a measure for tight junction-mediated barrier function. To confirm TLR2 involvement in barrier modulation by β2→1-fructans, ECIS experiments were repeated using TLR2 blocking antibody. After preincubation of T84 cells with short-chain β2→-fructans, the decrease in TEER as induced by PMA (62.3 ± 5.2%, P < 0.001) was strongly attenuated (15.2 ± 8.8%, P < 0.01). However, when PMA was applied first, no effect on recovery was observed during addition of the fructans. By blocking TLR2 on the T84 cells, the protective effect of short-chain β2→-fructans was substantially inhibited. Stimulation of human embryonic kidney human TLR2 reporter cells with β2→-fructans induced activation of nuclear factor kappa-light-chain-enhancer of activated B cells, confirming that β2→-fructans are specific ligands for TLR2. To conclude, β2→-fructans exert time-dependent and chain length-dependent protective effects on the T84 intestinal epithelial cell barrier mediated via TLR2. These results suggest that TLR2 located on intestinal epithelial cells could be a target of β2→-fructan-mediated health effects. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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