Autor: |
Wang, Shuang, Sun, Lei, Wei, Dan, Zhou, Baoku, Zhang, Junzheng, Gu, Xuejia, Zhang, Lei, Liu, Ying, Li, Yidan, Guo, Wei, Jiang, Shuang, Pan, Yaqing, Wang, Yufeng |
Zdroj: |
Journal of Microbiology; Jul2014, Vol. 52 Issue 7, p548-553, 6p |
Abstrakt: |
An alkaliphilic, moderately halophilic, bacterium, designated strain X10-1, was isolated from saline-alkaline soil inDaqing, Heilongjiang Province, China. Strain X10-1 was determined to be a Gram-positive aerobe with rod-shaped cells. The isolate was catalase-positive, oxidase-negative, non-motile, and capable of growth at salinities of 0-16% (w/v) NaCl (optimum, 3%). The pHrange for growth was 7.5-11.0 (optimum, pH 10.0). The genomic DNA G+C content was 47.7 mol%. Itsmajor isoprenoid quinone was MK-7 and its cellular fatty acid profile mainly consisted of anteiso-C, anteiso-C, iso-C, C, and iso-C. The peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid. The predominant polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, and phosphatidylglycerol. Phylogenetic analysis based on 16S rRNA gene sequences showed that X10-1 is a member of the genus Bacillus, being most closely related to B. saliphilus DSM15402 (97.8% similarity) and B. agaradhaerens DSM 8721 (96.2%). DNA-DNA relatedness to the type strains of these species was less than 40%. On the basis of the phylogenetic, physiological, and biochemical data, strain X10-1 represents a novel species of the genus Bacillus, for which the name Bacillus daqingensis sp. nov. is proposed. The type strain is X10-1 (=NBRC 109404 = CGMCC 1.12295). [ABSTRACT FROM AUTHOR] |
Databáze: |
Complementary Index |
Externí odkaz: |
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